— When first described the concept that a solution with a specific gravity higher than that of the eggs or other parasite elements present in feces could be used to concentrate them on the surface of the fluid was a pioneering development in parasitology see Koutz 1941 There appeared to be almost endless possibilities in the number of
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The faecal concentration methods based on sedimentation of parasites are useful for recovering heavy parasite eggs due to the concentration of the organisms in the sediment whereas the centrifugal flotation in zinc sulphate 9 is used preferentially to recover protozoa cysts because of the low density of parasitic forms as compared to the salt
The result that the combined zinc sulfate sedimentation flotation method SG as flotation medium provides diagnostic certainty only as of 80 epg has to be considered at preventing zoonoses If pet owners wish to prevent any zoonotic infection "zero tolerance" a monthly anthelminthic treatment should be advised instead of monthly fecal
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Home > Medical Reference and Training Manuals > > Concentration Techniques Parasitology I Buffered Methylene Blue Parasitology I Brine flotation Parasitology I Parasitology I Page Navigation Reagent zinc sulfate solution specific gravity to Add 331 grams of zinc sulfate
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The concentration procedures in general use in most clinical parasitology laboratories are zinc sulfate flotation 2 and Formalin ether sedimentation 4 The zinc sulfate procedure is reliable for detection of most eggs cysts and larvae but the Formalin ether method is thought to provide certain advantages including less distortion of
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The faecal concentration methods based on sedimentation of parasites are useful for recovering heavy parasite eggs due to the concentration of the organisms in the sediment whereas the centrifugal flotation in zinc sulphate 9 is used preferentially to recover protozoa cysts because of the low density of parasitic forms as compared to the salt
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Preparation of Flotation Solutions Mix salt in distilled water using a heat block and magnetic stirrer until no more salt will dissolve Allow to cool and any remaining undissolved salt to sediment in the bottle prior to use For Sheather s sucrose formaldehyde or phenol can be added to a concentration of % for preservation
The result that the combined zinc sulfate sedimentation flotation method SG as flotation medium provides diagnostic certainty only as of 80 epg has to be considered at preventing zoonoses If pet owners wish to prevent any zoonotic infection "zero tolerance" a monthly anthelminthic treatment should be advised instead of monthly fecal
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— The FE and the zinc sulfate flotation methods were compared in 2 studies which found them equally sensitive Bartlett et al 1978 Ritchie et al 1952 The use of ether has since been substituted with ethylacetate without any change in sensitivity Erdman 1981 to become the FEA concentration technique used in this study
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— The concentration procedures that we evaluated were the FAC FTC FGC and FEC Comparison of formalin ethyl ether sedimentation formalin ethyl acetate sedimentation and zinc sulfate flotation techniques for detection of intestinal parasites J Clin Microbiol 13 1981 pp 882 884
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— Background Intestinal parasitoses still are a noticeable threat to public health The direct diagnosis of such parasites requires the use of concentration techniques whose sensitivities for protozoan cysts and helminth eggs are far from equal Aim To compare the Willis Ritchie and Bailenger concentration techniques in terms of parasite
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— Our recommendation for the concentration method is that approximately 1 gram or a pea sized amount of faeces should be concentrated and that 10% formalin in water should be used instead of formalin in saline A surfactant Triton X 100 should be added when using ethyl acetate as a solvent The most effective concentration of Triton X
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— Results showed that centrifugation with ZnSO4 solution was significantly more likely to detect a positive sample than benchtop procedures due primarily to increased detection of Trichuris eggs and Giardia cysts by centrifugal flotation Fifty canine fecal samples were evaluated by five flotation procedures to compare the sensitivity of the zinc
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— Tests in the clinical parasitology section with the exception of rapid immunoassays are manual are time consuming and require personnel expertise The zinc sulfate flotation technique allows separation of most parasites from fecal debris The high specific gravity of the solution floats the organisms and examination of the top
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— Formalin ethyl ether sedimentation Formalin ethyl acetate sedimentation and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens
— Flotation techniques FLOTAC and mini FLOTAC for detecting gastrointestinal EPG were recorded with the flotation solution FS #7 zinc sulfate; specific gravity SG= at 1 20 and 1 25 dilution than other methods By contrast for the nematode Trypanoxyuris spp the best results were recorded with
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— Centrifugation recovers 3 5 times as many eggs over other passive floatation techniques such as the Ovassay® Passive flotation is especially poor at floating whipworms missing almost 2/3 of all whipworm infections completely We advocate the use of centrifugal fecal flotation as part of a comprehensive parasite control plan for dogs and
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— Three modifications of the McMaster method were compared in this study—the McMaster technique modified by Wetzel and Zajíček and the concentration McMaster technique according to Roepstorff and Nansen These modifications differ in the weights of faeces examined dilution factors used the presence/absence of additional
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— The concentration procedures that we evaluated were the FAC FTC FGC and FEC of formalin ethyl ether sedimentation formalin ethyl acetate sedimentation and zinc sulfate flotation techniques for detection of intestinal parasites J Clin Microbiol 131981882884
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The concentration procedures that constitute the main techniques applied in routine research and in parasitological kits are a spontaneous sedimentation; b centrifugation sedimentation with formalin ethyl acetate; and c flotation with zinc sulfate solution
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— Previous epidemiological studies with Toxocara species T canis/T cati by Childs [2] and Uga et al [3] used a flotation method to recover the eggs from living environments such as backyards and sandpits In these studies Childs used the standard zinc sulfate concentration procedures and Uga et al used their own modified flotation
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